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题名/责任者:

Gene knockout protocols = 基因敲除试验指南 / edited by Ralf Kühn, Wolfgang Wurst.

版本说明:

2nd ed.

出版发行项:

北京 : 科学出版社, c2012.

ISBN:

9787030343109 :

载体形态项:

xvi, 516 p. : ill. ; 27 cm.

变异题名:

基因敲除试验指南

丛编说明:

实验室解决方案

附加个人名称:

Kühn, Ralf.

附加个人名称:

Wurst, Wolfgang, 1955-

论题主题:

Transgenic mice.

论题主题:

Gene targeting.

论题主题:

Gene Knockout Techniques-methods-Laboratory Manuals.

中图法分类号:

Q75

一般附注:

“科爱传播.导读版”--Cover.

书目附注:

Includes bibliographical references and index.

内容附注:

Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst -- Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu -- Gene-trap vectors and mutagenesis / Silke De-Zolt ... [et al.] -- Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley -- Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts ... [et al.] -- Generation of shRNA transgenic mice / Christiane Hitz ... [et al.] -- Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti -- Gene targeting in mouse embryonic stem cells / Lino Tessarollo ... [et al.] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder ... [et al.] -- Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama -- Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo -- Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka ... [et al.] -- VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara ... [et al.] -- Generation of Cre recombinase-expressing trangenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz -- Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil -- Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts -- Transposon mutagenesis in mice / David A. Largaespada -- Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann -- Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis -- Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman -- Pathologic phenotyping of mutant mice / Roderick T. Bronson -- Systemic first-line phenotyping / Valérie Gailus-Durner ... [et al.]

原版附注:

Reprint. Originally published: New York, NY : Humana Press ; London : Springer [distributor], c2009. 9781934115268 (hbk. : alk. paper)

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