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- 题名/责任者:
- Plant fungal pathogens : methods and protocols / edited by Melvin D. Bolton, Bart P.H.J. Thomma.
- 出版发行项:
- New York : Humana Press, c2012.
- ISBN:
- 9781617795008 (alk. paper) :
- ISBN:
- 1617795003 (alk. paper)
- ISBN:
- 9781617795015 (e-ISBN)
- ISBN:
- 1617795011 (e-ISBN)
- 载体形态项:
- xvi, 648 p. : ill. (some col.) ; 26 cm.
- 丛编说明:
- Methods in molecular biology, 1940-6029 ; 835
- 丛编说明:
- Springer protocols
- 丛编统一题名:
- Methods in molecular biology (Clifton, N.J.) ; v. 835.
- 附加个人名称:
- Bolton, Melvin D. (Melvin Dennis), 1977-
- 附加个人名称:
- Thomma, Bart P. H. J.
- 中图法分类号:
- S432.4
- 书目附注:
- Includes bibliographical references and index.
- 内容附注:
- Fungal ABC transporter deletion and localization analysis -- Targeted gene replacement in fungal pathogens via agrobacterium tumefaciens- mediated transformation -- Activity-based protein profiling of infected plants -- The use of agroinfiltration for transient expression of plant resistance and fungal effector proteins in nicotiana benthamiana leaves -- Proteomic techniques for plant-fungal interactions -- Identification of HR-inducing cDNAs from plant pathogens via a Gateway((r))-compatible binary potato virus X-expression vector -- Freeze-fracture of infected plant leaves in ethanol for scanning electron microscopic study of fungal pathogens -- Real-time PCR for the quantification of fungi in planta -- Using non-homologous end-joining-deficient strains for functional gene analyses in filamentous fungi -- Atomic force microscopy: A tool for studying biophysical surface properties underpinning fungal interactions with plants and substrates -- Use of the yeast two-hybrid system to identify targets of fungal effectors -- Characterization of plant-fungal interactions involving necrotrophic effector-producing plant pathogens -- Heterologous production of fungal effectors in pichia pastoris -- The application of laser microdissection to profiling fungal pathogen gene expression in planta -- Metabolomics protocols for filamentous fungi -- Targeted gene replacement in fungi using a split-marker approach -- Massively parallel sequencing technology in pathogenic microbes -- Confocal microscopy in plant-pathogen interactions -- The use of open source bioinformatics tools to dissect transcriptomic data -- Population biology of fungal plant pathogens -- Polyethylene glycol (PEG)-mediated transformation in filamentous fungal pathogens -- In vitro induction of infection-related hyphal structures in plant pathogenic fungi -- Fungicide resistance assays for fungal plant pathogens -- Identification of lipid-binding effectors -- In silico identification and characterization of effector catalogs -- Horizontal transfer of supernumerary chromosomes in fungi -- The induction of mycotoxins by trichothecene producing fusarium species -- Identifying genes in fusarium verticillioides through forward and reverse genetics -- Assessment of autophagosome formation by transmission electron microscopy -- Fungal plant pathogen detection in plant and soil samples using DNA macroarrays -- Random insertional mutagenesis in fungal genomes to identify virulence factors -- A yeast secretion trap assay for identification of secreted proteins from eukaryotic phytopathogens and their plant hosts -- Comparing fungal genomes: Insight into functional and evolutionary processes -- Multigene phylogenetic analyses to delimit new species in fungal plant pathogens -- MAP kinase phosphorylation and cAMP assessment in fungi -- A one-step affinity-purification protocol to purify NB-LRR immune receptors from plants that mediate resistance to fungal pathogens -- Karyotyping methods for fungi -- Isolation of apoplastic fluid from leaf tissue by the vacuum infiltration-centrifugation technique -- Gene cloning using degenerate primers and genome walking -- Construction of hairpin RNA-expressing vectors for RNA-mediated gene silencing in fungi -- An unbiased method for the quantitation of disease phenotypes using a custom-built macro plugin for the program imageJ.
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