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- 题名/责任者:
- Protein engineering / edited by Dan E. Robertson, Joseph P. Noel.
- 出版发行项:
- Amsterdam ; Boston : Elsevier Academic Press, c2004.
- ISBN:
- 0121827933 :
- 载体形态项:
- xxxvii, 413 p., [16] p. of plates : ill. (some col.) ; 24 cm.
- 丛编题名:
- Methods in enzymology ; v. 388
- 附加个人名称:
- Robertson, Dan E.
- 附加个人名称:
- Noel, Joseph P.
- 论题主题:
- Genetic engineering.
- 论题主题:
- Proteins-Structure-activity relationships.
- 论题主题:
- Genetic Engineering.
- 论题主题:
- Protein Conformation.
- 论题主题:
- Proteins-genetics.
- 论题主题:
- Protein engineering.
- 论题主题:
- Proteins-Synthesis.
- 中图法分类号:
- Q55-5
- 中图法分类号:
- TQ93
- 书目附注:
- Includes bibliographical references and indexes.
- 内容附注:
- Section I. Methodology -- Gene site saturation mutagenesis : a comprehensive mutagenesis approach -- Single-stranded DNA family shuffling -- Module shuffling -- SCHEMA-guided protein recombination -- Staggered extension process in vitro DNA recombination -- Using incremental truncation to create libraries of hybrid enzymes -- Random multirecombinant polymerase chain reaction -- Gene library synthesis by structure-based combinatorial protein engineering -- New enzymes from combinatorial library modules -- Construction of protein fragment complementation libraries using incremental truncation -- Section II. Applications: optimization and screening -- GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform -- High throughput microplate screens for directed protein evolution -- Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities -- Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis -- Screening for oxidative resistance -- Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes -- Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system -- Section III. Applications: directed evolution of enzymatic function -- Directed evolution of lipases and esterases -- Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium -- Directed evolution of aldolases -- Changing the enantioselectivity of enzymes by directed evolution -- Control of stereoselectivity in phosphotriesterase -- Section IV. Applications: evolution of biosynthetic pathways -- Manipulation and analysis of polyketide synthases -- Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases -- Engineering carotenoid biosynthetic pathways -- Section V. Devices, antibodies, and vaccines -- Directed evolution of bacteriorhodopsin for device applications -- Engineering antibody affinity by yeast surface display -- A conditionally replicating virus as a novel approach toward an HIV vaccine.
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