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MARC状态:审校 文献类型:西文图书 浏览次数:79

题名/责任者:
Protein microarrays : methods and protocols / edited by Ulrike Korf.
出版发行项:
New York : Humana Press, c2011.
ISBN:
9781617792854 :
ISBN:
1617792853
载体形态项:
xi, 398 p. : ill. (some col.) ; 26 cm.
丛编说明:
Springer protocols
丛编说明:
Methods in molecular biology, 1064-3745 ; 785
丛编统一题名:
Methods in molecular biology (Clifton, N.J.) ; 785.
附加个人名称:
Korf, Ulrike.
论题主题:
Protein microarrays.
论题主题:
Protein Array Analysis.
中图法分类号:
Q51-33
书目附注:
Includes bibliographical references and index.
内容附注:
Reverse phase protein microarrays for clinical applications -- Impact of blocking and detection chemistries on antibody performance for reverse phase protein arrays -- Phosphoprotein stability in clinical tissue and its relevance for reverse phase protein microarray technology -- Utilization of RNAi to validate antibodies for reverse phase protein arrays -- Antibody-mediated signal amplification for reverse phase protein array-based protein quantification -- Reverse-phase protein lysate microarray (RPA) for the experimental validation of quantitative protein network models -- Characterization of kinase inhibitors using reverse phase protein arrays -- Use of formalin-fixed and paraffin-embedded tissues for diagnosis and therapy in routine clinical settings -- Producing reverse phase protein microarrays from formalin-fixed tissues -- Use of reverse phase protein microarrays to study protein expression in leukemia: Technical and methodological lessons learned -- Antibody microarrays as tools for biomarker discovery -- Assessment of antibody specificity using suspension bead arrays -- Quantitative analysis of phosphoproteins using microspot immunoassays -- Robust protein profiling with complex antibody microarrays in a dual-colour mode -- High-throughput studies of protein glycoforms using antibody-lectin sandwich arrays -- Microspot immunoassay-based analysis of plasma protein profiles for biomarker discovery strategies -- Recombinant antibodies for the generation of antibody arrays -- Producing protein microarrays from DNA microarrays -- Cell arrays and high-content screening -- Probing calmodulin protein-protein interactions using high-content protein arrays -- Protein function microarrays for customised systems-oriented proteome analysis -- Optimized autoantibody profiling on protein arrays -- Inkjet printing for the production of protein microarrays -- Impact of substrates for probe immobilization -- Contact printing of protein microarrays.
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