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- 题名/责任者:
- High throughput protein expression and purification : methods and protocols / edited by Sharon A. Doyle.
- 出版发行项:
- Totowa, N.J. : Humana Press ; [London : Springer, distributor], c2009.
- ISBN:
- 1588298795 (hbk. : alk. paper)
- ISBN:
- 9781597451963 (ebk.)
- ISBN:
- 1597451967 (ebk.)
- ISBN:
- 9781588298799 (hbk. : alk. paper) :
- 载体形态项:
- xii, 322 p., [4] p. of plates : ill. (some col.) ; 27 cm.
- 丛编说明:
- Methods in molecular biology ; 498
- 丛编统一题名:
- Methods in molecular biology (Clifton, N.J.) ; 498.
- 附加个人名称:
- Doyle, Sharon A. (Sharon Ann)
- 论题主题:
- Proteins-isolation & purification.
- 论题主题:
- Gene Expression.
- 论题主题:
- Proteomics-methods.
- 论题主题:
- Proteins-Separation-Methodology.
- 论题主题:
- Proteins-genetics.
- 中图法分类号:
- Q51-3
- 书目附注:
- Includes bibliographical references and index.
- 内容附注:
- High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning / Paul G. Blommel ... [et al.] -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.] -- "System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Nip2+s-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.].
- 内容附注:
- Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh -- PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood -- High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina -- High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.] -- High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee -- The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens -- High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi -- Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.] -- Expression and purification of soluble His?6s-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh -- High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery.
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