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- 000 02154cam a2200325 a 4500
- 008 130712s2014 nyua bf 001 0 eng
- 020 __ |a 9781936113002 (hardback) : |c CNY1885.00
- 020 __ |a 9781936113019 (paper)
- 040 __ |a DLC |b eng |c DLC |e rda |d DLC
- 050 00 |a QL737.R6 |b M2468 2014
- 082 00 |a 616.02/7333 |2 23
- 090 __ |a Q959.837-33/MME/(4)/ED-11
- 093 __ |a Q959.837-33 |2 5
- 099 __ |a CAL 022014030743
- 245 00 |a Manipulating the mouse embryo : |b a laboratory manual / |c Richard Behringer ... [et al.].
- 260 __ |a Cold Spring Harbor, New York : |b Cold Spring Harbor Laboratory Press, |c c2014.
- 300 __ |a xxii, 814 p. : |b ill. (some col.) ; |c 29 cm.
- 504 __ |a Includes bibliographical references and index.
- 505 0_ |a Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab.
- 650 _0 |a Mice |x Embryos |v Laboratory manuals.
- 650 _0 |a Mice |x Genetic engineering |v Laboratory manuals.
- 650 _0 |a Transgenic mice |v Laboratory manuals.
- 650 _0 |a Mice as laboratory animals.
- 700 1_ |a Behringer, Richard.