机读格式显示(MARC)
- 000 03488cam a2200337 a 4500
- 008 120910r20122009cc a b 001 0 eng d
- 020 __ |a 9787030343109 : |c CNY150.00
- 050 _4 |a QH506 |b .M45 v.530
- 099 __ |a CAL 022012096391
- 245 00 |a Gene knockout protocols = |b 基因敲除试验指南 / |c edited by Ralf Kühn, Wolfgang Wurst.
- 260 __ |a 北京 : |b 科学出版社, |c c2012.
- 300 __ |a xvi, 516 p. : |b ill. ; |c 27 cm.
- 500 __ |a “科爱传播.导读版”--Cover.
- 504 __ |a Includes bibliographical references and index.
- 505 0_ |a Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst -- Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu -- Gene-trap vectors and mutagenesis / Silke De-Zolt ... [et al.] -- Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley -- Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts ... [et al.] -- Generation of shRNA transgenic mice / Christiane Hitz ... [et al.] -- Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti -- Gene targeting in mouse embryonic stem cells / Lino Tessarollo ... [et al.] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder ... [et al.] -- Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama -- Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo -- Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka ... [et al.] -- VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara ... [et al.] -- Generation of Cre recombinase-expressing trangenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz -- Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil -- Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts -- Transposon mutagenesis in mice / David A. Largaespada -- Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann -- Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis -- Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman -- Pathologic phenotyping of mutant mice / Roderick T. Bronson -- Systemic first-line phenotyping / Valérie Gailus-Durner ... [et al.]
- 534 __ |p Reprint. Originally published: |c New York, NY : Humana Press ; London : Springer [distributor], c2009. |z 9781934115268 (hbk. : alk. paper)
- 650 _0 |a Transgenic mice.
- 650 _0 |a Gene targeting.
- 650 12 |a Gene Knockout Techniques |x methods |v Laboratory Manuals.
- 700 1_ |a Wurst, Wolfgang, |d 1955-