机读格式显示(MARC)
- 000 03438nam 2200373 a 4500
- 008 040812s2004 ne af b 001 0 eng d
- 020 __ |a 0121827933 : |c CNY1000.00
- 040 __ |a LHL |c LHL |d VVC |d MBU |d WJ7 |d OCLCQ |d CUX
- 099 __ |a CAL 022005007220
- 245 00 |a Protein engineering / |c edited by Dan E. Robertson, Joseph P. Noel.
- 260 __ |a Amsterdam ; |a Boston : |b Elsevier Academic Press, |c c2004.
- 300 __ |a xxxvii, 413 p., [16] p. of plates : |b ill. (some col.) ; |c 24 cm.
- 440 _0 |a Methods in enzymology ; |v v. 388
- 504 __ |a Includes bibliographical references and indexes.
- 505 0_ |a Section I. Methodology -- Gene site saturation mutagenesis : a comprehensive mutagenesis approach -- Single-stranded DNA family shuffling -- Module shuffling -- SCHEMA-guided protein recombination -- Staggered extension process in vitro DNA recombination -- Using incremental truncation to create libraries of hybrid enzymes -- Random multirecombinant polymerase chain reaction -- Gene library synthesis by structure-based combinatorial protein engineering -- New enzymes from combinatorial library modules -- Construction of protein fragment complementation libraries using incremental truncation -- Section II. Applications: optimization and screening -- GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform -- High throughput microplate screens for directed protein evolution -- Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities -- Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis -- Screening for oxidative resistance -- Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes -- Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system -- Section III. Applications: directed evolution of enzymatic function -- Directed evolution of lipases and esterases -- Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium -- Directed evolution of aldolases -- Changing the enantioselectivity of enzymes by directed evolution -- Control of stereoselectivity in phosphotriesterase -- Section IV. Applications: evolution of biosynthetic pathways -- Manipulation and analysis of polyketide synthases -- Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases -- Engineering carotenoid biosynthetic pathways -- Section V. Devices, antibodies, and vaccines -- Directed evolution of bacteriorhodopsin for device applications -- Engineering antibody affinity by yeast surface display -- A conditionally replicating virus as a novel approach toward an HIV vaccine.
- 650 _0 |a Genetic engineering.
- 650 _0 |a Proteins |x Structure-activity relationships.
- 650 _2 |a Genetic Engineering.
- 650 _2 |a Protein Conformation.
- 650 _2 |a Proteins |x genetics.
- 650 _0 |a Protein engineering.
- 650 _0 |a Proteins |x Synthesis.
- 700 1_ |a Robertson, Dan E.
- 700 1_ |a Noel, Joseph P.
- 905 __ |a CAU |f Q55-5/ME/V.388 |b W0073242(or.)
- 907 __ |a CAU |f Q55-5/ME/V.388 |b W0073242(or.)
- 999 __ |t C |A xy1 |a 20050221 16:39:37 |I xy1 |i 20050221 16:44:09 |G wzhlc |g 20050331 11:41:0