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- 000 03938cam 2200457 a 4500
- 008 081029s2009 nyua b 001 0 eng
- 020 __ |a 1934115266 (hbk.: alk. paper)
- 020 __ |a 9781597454711 (e-ISBN)
- 020 __ |a 1597454710 (e-ISBN)
- 020 __ |a 9781934115268 (hbk. : alk. paper) : |c CNY849.00
- 040 __ |a UKM |c UKM |d BTCTA |d YDXCP |d NLM |d CDX |d CUS |d COU |d BWX |d PAU
- 050 _4 |a QH506 |b .M45 v.530 2009
- 060 10 |a QH 442.3 |b G326 2009
- 060 00 |a W1 |b ME9616J v.530 2009
- 099 __ |a CAL 022009092263
- 245 00 |a Gene knockout protocols / |c edited by Ralf Küh n, Wolfgang Wurst.
- 260 __ |a New York, NY : |b Humana Press, |c c2009.
- 300 __ |a xvi, 516 p., [4] p. of plates : |b ill. (some col.) ; |c 27 cm.
- 490 1_ |a Methods in molecular biology, |x 1064-3745 ; |v v. 530
- 490 0_ |a Springer protocols
- 500 __ |a Previous ed.: 2001.
- 504 __ |a Includes bibliographical references and index.
- 505 0_ |a Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst -- Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu -- Gene-trap vectors and mutagenesis / Silke De-Zolt ... [et al.] -- Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley -- Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts ... [et al.] -- Generation of shRNA transgenic mice / Christiane Hitz ... [et al.] -- Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti -- Gene targeting in mouse embryonic stem cells / Lino Tessarollo ... [et al.] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder ... [et al.] -- Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama -- Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo -- Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka ... [et al.] -- VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara ... [et al.] -- Generation of Cre recombinase-expressing trangenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz -- Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil -- Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts -- Transposon mutagenesis in mice / David A. Largaespada -- Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann -- Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis -- Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman -- Pathologic phenotyping of mutant mice / Roderick T. Bronson -- Systemic first-line phenotyping / Valérie Gailus-Durner ... [et al.]
- 650 12 |a Gene Knockout Techniques |x methods |v Laboratory Manuals.
- 650 _0 |a Transgenic mice.
- 650 _0 |a Gene targeting.
- 700 1_ |a Wurst, Wolfgang, |d 1955-
- 830 _0 |a Methods in molecular biology (Clifton, N.J.) ; |v v. 530.
- 905 __ |a CAU |f Q75/GKP/(2) |b W0085464ED-11(or.)
- 907 __ |a CAU |f Q75/GKP/(2) |b W0085464ED-11(or.)
- 999 __ |I xy1 |i 20101202 14:52:15 |G xy1 |g 20101209 10:43:1