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- 题名/责任者:
- Gene knockout protocols / edited by Ralf Küh n, Wolfgang Wurst.
- 版本说明:
- 2nd ed.
- 出版发行项:
- New York, NY : Humana Press, c2009.
- ISBN:
- 1934115266 (hbk.: alk. paper)
- ISBN:
- 9781597454711 (e-ISBN)
- ISBN:
- 1597454710 (e-ISBN)
- ISBN:
- 9781934115268 (hbk. : alk. paper) :
- 载体形态项:
- xvi, 516 p., [4] p. of plates : ill. (some col.) ; 27 cm.
- 丛编说明:
- Methods in molecular biology, 1064-3745 ; v. 530
- 丛编说明:
- Springer protocols
- 丛编统一题名:
- Methods in molecular biology (Clifton, N.J.) ; v. 530.
- 附加个人名称:
- Kühn, Ralf.
- 附加个人名称:
- Wurst, Wolfgang, 1955-
- 论题主题:
- Gene Knockout Techniques-methods-Laboratory Manuals.
- 论题主题:
- Transgenic mice.
- 论题主题:
- Gene targeting.
- 中图法分类号:
- Q75
- 一般附注:
- Previous ed.: 2001.
- 书目附注:
- Includes bibliographical references and index.
- 内容附注:
- Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst -- Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu -- Gene-trap vectors and mutagenesis / Silke De-Zolt ... [et al.] -- Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley -- Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts ... [et al.] -- Generation of shRNA transgenic mice / Christiane Hitz ... [et al.] -- Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti -- Gene targeting in mouse embryonic stem cells / Lino Tessarollo ... [et al.] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder ... [et al.] -- Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama -- Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo -- Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka ... [et al.] -- VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara ... [et al.] -- Generation of Cre recombinase-expressing trangenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz -- Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil -- Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts -- Transposon mutagenesis in mice / David A. Largaespada -- Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann -- Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis -- Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman -- Pathologic phenotyping of mutant mice / Roderick T. Bronson -- Systemic first-line phenotyping / Valérie Gailus-Durner ... [et al.]
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