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题名/责任者:

Manipulating the mouse embryo : a laboratory manual / Richard Behringer ... [et al.].

版本说明:

4th ed.

出版发行项:

Cold Spring Harbor, New York : Cold Spring Harbor Laboratory Press, c2014.

ISBN:

9781936113002 (hardback) :

ISBN:

9781936113019 (paper)

载体形态项:

xxii, 814 p. : ill. (some col.) ; 29 cm.

附加个人名称:

Behringer, Richard.

论题主题:

Mice-Embryos-Laboratory manuals.

论题主题:

Mice-Genetic engineering-Laboratory manuals.

论题主题:

Transgenic mice-Laboratory manuals.

论题主题:

Mice as laboratory animals.

中图法分类号:

Q959.837-33

书目附注:

Includes bibliographical references and index.

内容附注:

Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab.

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